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翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Single particle analysis ： ウィキペディア英語版 Single particle analysis Single Particle Analysis is a group of related computerized image processing techniques used to analyze images from transmission electron microscopy (TEM). These methods were developed to improve and extend the information obtainable from TEM images of particulate samples, typically proteins or other large biological entities such as viruses. Individual images of stained or unstained particles are very noisy, and so hard to interpret. Combining several digitized images of similar particles together gives an image with stronger and more easily interpretable features. An extension of this technique uses single particle methods to build up a three-dimensional reconstruction of the particle. Using cryo-electron microscopy it is now possible to generate reconstructions with sub-nanometer resolution and near-atomic resolution in the case of highly symmetric viruses. ==Techniques== Single particle analysis can be done on both negatively stained and vitreous ice-embedded cryo-EM samples. Single particle analysis methods are, in general, reliant on the sample being homogeneous, although techniques for dealing with conformational heterogeneity are being developed. Images (micrographs) collected on film are digitized using high-quality scanners, although increasingly electron microscopes have built-in CCD detectors coupled to a phosphorescent layer. The image processing is carried out using specialized software programs (for instance 〔http://www.femtoscanonline.com/wiki/en/processing/%D0%B0%D0%BD%D0%B0%D0%BB%D0%B8%D0%B7_%D0%B2%D1%8B%D0%B4%D0%B5%D0%BB%D0%B5%D0%BD%D0%BD%D1%8B%D1%85_%D0%BE%D0%B1%D0%BB%D0%B0%D1%81%D1%82%D0%B5%D0%B9〕), often run on multi-processor computer clusters. Depending on the sample or the desired results, various steps of two- or three-dimensional processing can be done. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Single particle analysis」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.